8-Quinolinesulfonyl chloride as a coupling agent in oligonucleotide synthesis via phosphotriester approach
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چکیده
منابع مشابه
Oligonucleotide synthesis using the manual phosphotriester method.
During the past few years, synthetic DNA, used as a primer in DNA polymerization, in site-directed mutagenesis or as a probe in gene selection, has assumed a central role in recombinant DNA technology (1). This was made possible by the development of methods for efficient solid phase chemical synthesis. Deoxyribonucleotides are ideally suited to solid-phase synthesis since their relative chemic...
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Evidence will be presented to show that the allyl group is suitable for the protection of a 3'-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a phosphotriester approach, of two cyclic tetraribonucleotides [r(AAAA) and r(UAMe2UAMe2)], two cyclic hexadeoxyribonucleotides [d(CGCGCG) and d(TAAAAA)] and a cyclic octadeoxyribonucleotide [d(CGTGCGTG)].
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1978
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/1.suppl_2.s345